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Single-cell suspensions were diluted, centrifuged, washed once with PBS/DTT and twice with PBS, before cryopreservation (F-media, 40% FBS, 10% DMSO)

studies have found that consumers are largely unaware of the negative health consequences of cigarette smoking not mentioned in current warnings as well as more specific information about the negative health effects and their mechanisms (Refs

Mol Cell Biol 32:840851 Newman DJ, Cragg GM (2012) Natural products as sources of new drugs over the 30 years from 1981 to 2010

Additionally, while a 10 percent increase in exposure to anti-smoking messaging was associated with a 12 percent increase in quit attempts among those who did not hold the weight-control belief, no increase in quit attempts was reported by smokers who did believe so